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Verification of differentially expressed genes (DEG) in ILP signaling pathway by qRT-PCR.

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posted on 06.07.2017, 17:37 by Benfeng Han, Shen Zhang, Fanrong Zeng, Jianjun Mao

(A) DEG results from transcriptome analysis. The fold change of gene was calculated as log2 value of each fed/starved comparison and shown on y-axis. (B) qRT-PCR results of gene expression. Values are expressed as Mean±SE of three independent replicates.

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Nutritional and reproductive signaling revealed by comparative gene expression analysis in Chrysopa pallens (Rambur) at different nutritional statuses
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Illumina short-read sequencingcarbon metabolismChrysopa pallens Ramburinsulin-like peptidesinsulin receptorsendoplasmic reticulumcDNA amplificationgene expression profilesdifferentially expression genes1501 genesChrysopa pallensgene expression analysisstatuses BackgroundDEGprotein processing833 bp241 pathwayspredaceous insect species71236 unigenesKEGG orthology classification

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