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Verification of cloned SSB as a genuine protein (A) and complementation assay (B)

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posted on 2011-12-30, 23:53 authored by Gregor Witte, Claus Urbanke, Ute Curth

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Taken from "Single-stranded DNA-binding protein of : a biophysical characterization"

Nucleic Acids Research 2005;33(5):1662-1670.

Published online 21 Mar 2005

PMCID:PMC1069009.

© The Author 2005. Published by Oxford University Press. All rights reserved

Samples were boiled in 1% SDS sample buffer, proteins were separated in Tricin SDS–PAGE (), blotted on PVDF membrane and immunologically detected with a polyclonal rabbit anti-SSB serum. () Cell extract from (lane 1) compared with 200 ng purified recombinant SSB (lane 2). () Lane 3, RDP268 (pSF1-SSB) cells express only SSB; lane 4, RDP268 (pACYCssb) cells express only SSB; and lane 5, purified recombinant SSB (15 ng). Lane 3 and lane 4 contain equal amounts of cells.

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