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Validation of AS changes using semi-quantitative RT-PCR.

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posted on 09.11.2017, 19:01 authored by June H. Tan, Andrew G. Fraser

(A) Semi-quantitative RT-PCR reproduces AS changes observed by RNA-seq. Independent RNA samples isolated from L4-sorted worms were used to validate RNA-seq data. (B) Changes in PSI values in wild-type and mutant worms show strong correlation with RNA-seq data. PSI values estimated from RT-PCR data was compared to RNA-seq derived PSI values and the strength of correlation was measured using Pearson’s correlation co-efficient.