VPS13C knockdown sensitized cells to proteasome inhibition.
3T3-L1 fibroblasts engineered with the doxycycline-induced LacZ or VPS13C knockdown system were treated for three days without or with doxycycline in the presence of chloroquine, MG-132, or 3-MA. Cell viability was then analyzed using MTS assay. Results are representative of three experiments. Asterisks denote statistical significance.