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Upon CVB3 infection IFN-β induction in the liver is conferred primarily by hepatocytes.

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posted on 2018-08-03, 17:39 authored by Wolfgang Koestner, Julia Spanier, Tanja Klause, Pia-K. Tegtmeyer, Jennifer Becker, Vanessa Herder, Katharina Borst, Daniel Todt, Stefan Lienenklaus, Ingo Gerhauser, Claudia N. Detje, Robert Geffers, Martijn A. Langereis, Florian W. R. Vondran, Qinggong Yuan, Frank J. M. van Kuppeveld, Michael Ott, Peter Staeheli, Eike Steinmann, Wolfgang Baumgärtner, Frank Wacker, Ulrich Kalinke

(A and B) Lethally irradiated IFN-βwt/Δβ-luc (Δβ-luc) or C57BL/6 (B6) recipients were reconstituted with 1 x 107 BM cells from C57BL/6 (B6>Δβ-luc, red) or IFN-βwt/Δβ-luc mice (Δβ-luc>B6, blue), respectively. IFN-βwt/Δβ-luc recipients reconstituted with IFN-βwt/Δβ-luc BM were used as controls (Δβ-luc> Δβ-luc, black). Eight weeks after transplantation, mice were infected i.p. with 2 × 104 PFU CVB3 and imaged at 0, 2, or 4 dpi (n = 3–9). (A) One representative mouse of each group is shown. (B) Quantification of in vivo imaging by analysis of cervical and upper abdominal regions of interest (ROI). Values are mean + SD. (C-F) Conditional IFN-β reporter mice with an activated reporter in myeloid cells (LysM-Cre+/-IFN-βwt/floxβ-luc) or in hepatocytes (Alb-Cre+/-IFN-βwt/floxβ-luc) were infected as in (A) and imaged at the indicated dpi. IFN-βwt/Δβ-luc control mice with C57BL/6 albino or C57BL/6 background were used as controls, respectively. (C, E) One representative mouse of each genotype is shown. (D, F) Quantification of in vivo imaging (n = 3–9). Values are mean + SD. One-Way ANOVA test was used for statistical analysis, *P < 0.05; **P < 0.01; ***P < 0.001.