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Up-regulation of AGGF1 in response to acute MI and robust therapeutic effects of AGGF1 protein therapy on MI.

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posted on 2016-08-11, 17:32 authored by Qiulun Lu, Yufeng Yao, Zhenkun Hu, Changqing Hu, Qixue Song, Jian Ye, Chengqi Xu, Annabel Z. Wang, Qiuyun Chen, Qing Kenneth Wang

(A) Real-time reverse transcription (RT)-PCR analysis for AGGF1 mRNA expression in mice with LAD ligation or sham operation (Sham) (n = 6/group). Pre, before surgery. (B) Western blot analysis for AGGF1 protein expression (n = 6 mice/group). (C) Immunostaining analysis for AGGF1 protein expression in cross-sections of hearts (n = 5). Scale bar = 50 μm. (D) AGGF1 (500 ng/ml) induces autophagy in HUVECs under a hypoxic condition for different time points (n = 3/group). (E) AGGF1-specific siRNA inhibited autophagy under normoxia or hypoxia. (F) Post-MI survival of mice with AGGF1 treatment (n = 20), mice with IgG treatment (n = 30), and sham mice (n = 14). (G) Representative M-mode echocardiograms. (H) Effects of AGGF1 protein therapy on myocardial function and contraction. Underlying data are shown in S1 Data.