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Download fileU-ExM confirms the organisation of the actin cytoskeleton during P. falciparum gametocytogenesis.
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posted on 2022-01-25, 18:43 authored by Ravish Rashpa, Mathieu BrochetA-D. Representative full projections of P. falciparum gametocyte stages. α/β tubulin: magenta; amine reactive groups / NHS-ester: shades of grey; actin: cyan. Column 6 shows the 3D surface topology reconstruction of α/β-tubulin and actin. A and B. At early stages, a punctate distribution of actin is observed. C-D. From stage IV gametocytes, actin shows a polarised localisation, mostly concentrated at both extremities in tubulin-free areas showing a mesh-like organization. Actin also extends lengthwise alongside the subpellicular microtubules. Insets in (C and D) are close ups of both extremities. Scale bars = 5 μm.
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results provide insightsprotein kinase 1confers cell rigiditybulk proteome labellingsubpellicular microtubule networkdiv >< pde novo </eight basal bodiesbasal bodies displayalso assembles axonemesplasmodium falciparum </coordinate axoneme assemblydynamic architecture reliesbasal bodiesplasmodium </p </axoneme assemblymosquitoes reliesmicrotubule wallintranuclear bodiesdynamic movementbasal bodyberghei </axoneme formationupon activationtwo nonsequential nucleationremains elusiveproteinaceous filamentsmolecular organisationmolecular architecturemitotic spindlesmay reflectlike structuregametocyte stagesfunctional transitionfirst reconstructedexpansion microscopydependent mannercentral corescausing parasitescanonical regulators
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