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Tumor growth inhibition and on-target effect of RA414 in a syngeneic mouse model of ovarian cancer.

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posted on 10.09.2021, 17:36 by Ravi K. Anchoori, Logan George, Ssu-Hsueh Tseng, Brandon Lam, Srinidhi Polkampally, Anjali D. Amiano, Palmer Foran, Hannah Tsingine, Harideep Samanapally, Fernanda Carrizo Velasquez, Samarjit Das, Deyin Xing, Ahmad Bin Salam, Balasubramanyam Karanam, Chien-Fu Hung, Richard B. S. Roden

(A) C57BL/6 mice bearing intraperitoneal ID8-Vegf+ Defb29+ tumor that expresses luciferase was imaged using an IVIS200 at the indicated days following treatment with either vehicle (PEG400: Cremophor: Tween40) or RA414 (10 mg/Kg/every 3 days/ 3 doses total) and RA414 (3mg/Kg/every 3 days/total 9 doses). (B) From Fig A, two mice each from the vehicle and RA414 (3 mg/Kg) treated mice were euthanized and ascites collected at day 24. The ascites cells were subjected to Western blotting analysis using antibody to ubiquitin or, as a loading control, actin.