Tubastatin A, but not Aurora A Inhibitor, protects purified primary cortical neuron cultures from etoposide.
(A) Micrograph showing cultured primary cortical neurons are ciliated (arrowheads). Scale bars = 10 μm. (B) Representative images of primary cortical neuron cultures with and without AraC treatment showing proliferation of glial cells in the culture without treatment. Scale Bar = 100 μm. (C) Quantification of cell cultures with or without AraC treatment, showing a significantly higher proportion of GFAP-positive cells in cultures without AraC purification. Data presented as mean ± SEM. (D) Representative images of MTT crystallization in surviving cortical neurons after etoposide toxicity, in normal (untreated), DMSO control, or TBA treated cultures. (E) TBA treatment significantly increased cell survival after etoposide insult compared to DMSO control, but not in cultures that did not receive AraC. (F) Quantification of cortical neuron survival after AurA inhibitor I treatment showing increased cell death after etoposide injury.