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Trisomy fibroblasts showed increased oxidative stress and accumulation of damaged mitochondria.

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posted on 29.07.2019, 17:34 authored by Nobutoshi Nawa, Katsuya Hirata, Keiji Kawatani, Toshihiko Nambara, Sayaka Omori, Kimihiko Banno, Chikara Kokubu, Junji Takeda, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Daisuke Okuzaki, Hidetoshi Taniguchi, Hitomi Arahori, Kazuko Wada, Yasuji Kitabatake, Keiichi Ozono

(A) Relative ROS production as expressed by relative MitoSOX/MitoTracker ratio (n = 5–6 per cell line). *P < 0.05. Dip, diploid; Tri, trisomy. (B) Relative mitochondrial mass per cell as assessed by average TOM20-positive area per cell. The upper panels show representative phase contrast images in which mitochondria were stained with MitoTracker. Bar = 50 μm. The lower panel shows corresponding quantification (n = 3–8 per cell line). **P < 0.01. Dip, diploid; Tri, trisomy. (C) Relative mitochondrial membrane potential per cell (n = 5–10 per cell line). **P < 0.01. Dip, diploid; Tri, trisomy. Comparisons were made by Student’s t-test.

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