Treatment with spironolactone and eflornithine changes expression levels for a subset of differentiation associated genes.
Q-PCR experiment showing relative expression levels for indicated genes in parasites treated for 3 days with 33μM spironolactone, eflornithine, or vehicle control. Error bars indicate standard error of 3 biological replicates. *, **, *** indicates p < 0.05, p < 0.01, and p < 0.001 respectively as measured by an unpaired, two-sided Student’s t-test. Note, relative expression is plotted on a log scale.