Transient transfection of Env variants with highly negative diffsel does not induce UPR.
RT-PCR analysis of SEC24D, HSPA5, DNAJB9, and HYOU1 in HEK293T cells expressing GFP (negative control), wild-type Env, and 3 Env variants that were strongly negatively selected in +XBP1s versus basal (C54W, L111P, and L556R). As a positive control for UPR induction, HEK293T cells expressing GFP were treated with thapsigargin (Tg; 2 μM) for 6 h (GFP + Tg). RT-PCR data are presented as fold increase relative to GFP-transfected negative control. RT-PCR data values are provided in S7 Data.