Trajectory analysis of spatial domains using Monocle and Slingshot algorithms and autocorrelation analysis of marker gene for endothelial cell.

(A) The plot shows the Uniform Manifold Approximation and Projection (UMAP) representation of spatial domains, colored by their Leiden cluster assignments, overlaid with the trajectories predicted by the Monocle algorithm. The root point of the trajectory is labeled by the white node, and the branch points are labeled by the gray node. (B) The plot shows the UMAP representation of spatial domains, overlaid by their Leiden cluster assignments (left), overlaid with the trajectories predicted by the Slingshot algorithm (right). (C) Line plot depicting the relationship between Leiden clusters and Geary’s C for the genes PECAM1 (blue), VWF (orange), and CD93 (green).

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