Total (T) and soluble (S) expression for protein 1 – 30 (Table 1) with different C-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as Figure 2
Taken from "Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression"
BMC Biotechnology 2004;4():32-32.
Published online 14 Dec 2004
Copyright © 2004 Dyson et al; licensee BioMed Central Ltd.Expression plasmids employed were (g) pET-DEST42, (h) pDEST-C101 or pDEST-C102 with either (i) MBP, (j) GST, (k) GFP (l) Trx (m) Dhfr or (n) DHFR inserted between the DraIII and BfrBI sites as shown in Figure 1.