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Total (T) and soluble (S) expression for protein 1 – 30 (Table 1) with different C-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as Figure 2

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posted on 31.12.2011, 00:51 by Michael R Dyson, S Paul Shadbolt, Karen J Vincent, Rajika L Perera, John McCafferty

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Taken from "Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression"

BMC Biotechnology 2004;4():32-32.

Published online 14 Dec 2004

PMCID:PMC544853.

Copyright © 2004 Dyson et al; licensee BioMed Central Ltd.

Expression plasmids employed were (g) pET-DEST42, (h) pDEST-C101 or pDEST-C102 with either (i) MBP, (j) GST, (k) GFP (l) Trx (m) Dhfr or (n) DHFR inserted between the DraIII and BfrBI sites as shown in Figure 1.

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