Total RNA concentration of enriched plasma sEVs.
Isolation of sEVs was conducted by PEG-based methods SPEGT, DPEGT, and miRCURY kit and UC. (a) Effect of RNase A with or without proteinase k (PK) treatment on RNA yield. *** p < 0.001 as determined by Student’s t-test, n = 5. (b) Effect of single treatment (ST) and double treatment (DT) of PEG with different concentrations on total RNA yield. Data represent the mean ± SEM. * p < 0.05 as determined by Student’s t-test. (c) Total RNA yield upon 8% SPEGT, 8% DPEGT, miRCURY kit and UC. Data represent the mean ± SEM. ** p < 0.01, * p < 0.05 and ns (not significant) as determined by either Student’s t-test or ANOVA test.