To identify early selection markers for latex production in Hevea spp, nodal sections from species Hevea brasiliensis and Hevea spruceana subjected to different treatments of plant growth regulators (PGRs) were collected. After that, morphoanatomical structure, and expression the genes involved in latex biosynthesis (REF1, REF3, REF7, and SRPP1) and formation of laticiferous vessels (CDPK1, MPK3, and WOX13) were investigated.