Time-course immunofluorescence analysis of LDs, NS5A and Core in WT infected cells.

Huh7.5 cells were electroporated with an in vitro transcript of mJFH-1 WT. At the indicated h.p.e. cells were fixed and stained with anti-NS5A and Core antibodies, BODIPY 558/568-C_12, and DAPI and imaged by Airyscan microscopy (A). Spatial data for LDs were determined from 10 cells for each time point using Fuji. These data were used to determine the number of LDs per cell (B), the average size of LDs (C) and the distance of each LD from nucleus at different time points (D). **** indicates significant difference (P<0.0001) from the results for LDs in untransfected cells. The scale bars are 5μm and 0.5 μm, respectively.