Third harmonic generation imaging and analysis of the effect of low gravity on the lacuno-canalicular network of mouse bone - Fig 2

Comparison between confocal fluorescence microscopy (a,b,c) and THG imaging (d,e,f) of two different samples. (a,d) 2D composite images of full transverse cross-sections of a mouse femur. Regions shown in (b,e) are indicated by a green box in (a,d). Regions in (c,f) are indicated by a yellow box in (b,e). Red stars in (d) indicate the presence of air bubbles due to localized heating by absorption of the excitation light in the presence of polishing residues. Scale bar 500 μm (a,d), 20 μm (b,e) and 5 μm (c,f).