siRNA-mediated knockdown of GluN3A mRNA in 46C-derived NSCs.
46C-derived NSCs were either transfected with a mixture of scrambled siRNAs1+2 or with a mixture of siRNAs1+2 directed against GluN3A. A: qRT-PCRs were performed to analyse the knockdown of GluN3A at the mRNA level. GluN3A was significantly downregulated upon transfection with the mixture of siRNAs1+2 against GluN3A. There were no statistically significant differences in GluN3A expression between non-transfected NSCs and NSCs transfected with the mixture of scrambled siRNAs1+2 or scrambled siRNA2. Data represent means +/- SEM; statistical significances were assigned by unpaired Student’s t-test. ***p < 0.001. n = 3 independent experiments.