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The serum IgG levels in first-generation rats.

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posted on 2016-02-04, 21:23 authored by Rucheng Chen, Xiaoqiao Tang, Bolin Fan, Jiafa Liu, Xudong Jia, Xiaoguang Yang

Maternal PBS exposure was used in the experimental control rats(F0matPBS), and maternal OVA exposure was used in the F1 experimental rats(F0matOVA), which were randomly divided into the OVA and non-OVA groups, respectively. The OVA group and non-OVA groups were administered treatments daily for 6 weeks, and blood samples were taken on the 2nd, 4th, and 6th weeks from the orbital plexus. The number of animals per experiment was 32, and each group contained16 animals. The serum IgG levels were measured with an ELISA technique. Data are presented as P/N values representing the ratios of the OVA group OD values divided by the non-OVA group values in the respective experiment. P/N IgG values>2 were considered to indicate positive expression levels. The statistical analyses were performed with Non-parametric test(Mann-Whitney U), which compared the control and F1 rats. *p<0.05. (Data in S1 and S2 Tables)