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The role of Panx3 in AMPK and Smad signaling.

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posted on 11.05.2017 by Tsutomu Iwamoto, Takashi Nakamura, Masaki Ishikawa, Keigo Yoshizaki, Asuna Sugimoto, Hiroko Ida-Yonemochi, Hayato Ohshima, Masahiro Saito, Yoshihiko Yamada, Satoshi Fukumoto

(A) mDP cells were treated with 1 mM of Ara-a for 30 min before treatment with BMP2. 1 mM AICAR was tested Smad1/5/8 and AMPK phosphorylations by western blotting. Data were pooled from three independent experiments with error bars designating standard deviation of the mean. Statistical analysis was performed using analysis of variance (*P < 0.05). (B) The time course of Smad1/5/8 phosphorylation in mDP cells following treatment with AICAR was analyzed by western blotting using the anti-phospho-Smad1/5/8 antibody. (C) Panx3-transfected mDP cells were treated with either the Panx3 peptide or the calmodulin inhibitor W-7, and the phosphorylation of both Smad1/5/8 and AMPK was analyzed by western blotting. Data were pooled from three independent experiments with error bars designating standard deviation of the mean. Statistical analysis was performed using analysis of variance (*P < 0.05).

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