The role of CTCF in regulating ANGPLT4 transcription.
(A) qRT-PCR analysis of HepG2 cells transfected with the siRNAs (siCont, siCTCF, and siRAB11B AS) for 48 h and then treated with Dex (100 nM). Expression levels were normalized to those of 36B4 transcripts. (B) Western blot analysis of CTCF and GR expression in siRNA-transfected cells. Asterisks indicate statistically significance among siRNA-transfected cells at each time point. (C) qRT-PCR analysis of ANGPTL4 mRNA expression in siRNA-transfected HepG2 cells treated with Dex (see Fig 4A). (D) qRT-PCR analysis of ANGPTL4 mRNA expression in siRNA-transfected LTDT cells treated with Dex. Expression levels were normalized to those of 36B4 transcripts. (E) Enrichment of CTCF at AC3 and GR at AG sites in siRNA-transfected cells. ChIP-qPCR analysis was performed using anti-CTCF, anti-GR, and anti-rabbit IgG (control) antibodies, followed by quantitative PCR using primers specific for each site. Asterisks indicate statistically significance between control and CTCF-knockdown cells at each time point. *P < 0.05, **P < 0.01, ***P < 0.005.