The real-time measurement of the total resistance across HRECs under the effect of the Hexokinase inhibitor (Lonid; lonidamine).

(A) The plot illustrates the normalized resistance across HRECs over time, measured at an AC frequency of 4000 Hz for both the control group and HRECs treated with Lonid (1μM & 10 μM). The resistance was normalized using the measurement taken before the addition of the inhibitor, considered as t = 0 hour. (B) The bar graph represents the normalized resistance for the control group and HRECs treated with Lonid (1μM & 10 μM) at the endpoint of the experiment (t = 120 hours). (C) The bar graph displays the areas under the normalized resistance curve for the control group and HRECs treated with Lonid (1μM & 10 μM) during the time interval of t = 0–120 hours. n = 4 biological replicates for each group. Abbreviations: Norm (normalized), AUC (area under the curve), ns (no significance).