Representative flow cytometry data of healthy peripheral blood monocytes and MDM.

(A) SSC-A/FSC of harvested healthy peripheral blood monocytes. (B) SSC-A/FSC of unstimulated healthy peripheral blood MDM, generated after differentiation of monocytes with 100ng/mL M-CSF for 7 days. (C) SSC-A/PE-Cy7-A -CD68 staining of monocytes. (D) SSC-A/PE-Cy7-A -CD68 staining of unstimulated healthy human MDM. SSC-A = side scatter area (measure of cell granularity), FSC-A = forward scatter area (measure of cell size).

S1A and B Figs demonstrate clear increase in granularity of cells, consistent with monocyte differentiation to macrophages. S1C and D Figs demonstrate a clear increase in CD68 expression in macrophages (D) compared to monocytes (C), along with an increase in cell granularity, consistent with successful monocyte to macrophage differentiation.

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