The rpoZ and spoT genes are transcribed as a bicistronic operon.
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posted on 2024-04-04, 17:43 authored by Cristian Camilo Ortiz-Vasco, Soledad Moreno, Luis Alonso Quintero-Navarro, Juliana Berenice Rojo-Rodríguez, Guadalupe EspínThe non-polar mutation in UWrpoZ did not affect spoT transcription. (A) Physical map of the A. vinelandii rpoZ (Avin02820) and spoT (Avin02810) genes. The arrows indicate the oligonucleotides used to determine the transcript corresponding to the intergenic region of the rpoZ-spoT operon. (B) RT-PCR using total RNA from UW136 to amplify a 120 bp of the rpoZ-spoT intergenic region. Total DNA from UW136 was used as positive control. (C) Transcription of spoT in UWrpoZ compared with its parental UW136 strain, determined by RT-qPCR.
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wild type levelstwo sites withinstringent response regulatesstringent response exerteddiverse bacterial speciescontrol gene expressionescherichia coli azotobacter vinelandii turn activates transcriptionsubunits β ’div >< psite 2 locatedrpos protein levelssite 1optimal expressionrpoz rpos rela dksa proteinω (<transcriptomic analysissecondary channelrpos regulationrevealed downregulationprecursor substrateoptimal transcriptionnegative effectmutation reducedenzymes reladksa different mechanismsdespite expressingdata indicateconstitutive promoter
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