The frequency of nt variations versus nucleotide position in the gRNA.

Gray bars indicate the number of gRNA sequence classes with an identified nt difference between related procyclic and bloodstream gRNAs. Nucleotide numbering for each gRNA was normalized by setting the start of the Watson-Crick anchor region to zero. Black bars indicate the number of gRNA sequence classes whose contiguous Watson-Crick anchors end at that position (start of Watson-Crick = zero, so this is an indication of the length of the contiguous Watson-Crick region).