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The effect of global IL-4 neutralization on survival during neutropenia models of invasive pulmonary aspergillosis using NRG and Nlrx1-/- mice.

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posted on 21.09.2020, 17:46 by Bridget Kastelberg, Nuria Tubau-Juni, Tariq Ayubi, Austin Leung, Andrew Leber, Raquel Hontecillas, Josep Bassaganya-Riera, Shiv D. Kale

(A) Survival study post adoptive transfer of Nlrx1-/- CD103+ dendritic cells into NRG mice depleted of neutrophils via Ly6G+ depletion (1A8 Clone) and IL-4 neutralization (IL-4 Neut., Nlrx1-/- → NRG) or isotype control (Nlrx1-/- → NRG) antibody one day prior and three days post inoculation with AF293 conidia via non-invasive intratracheal instillation (107 conidia/ 25μL). (B) Survival study for Nlrx1-/- mice depleted of neutrophils via Ly6G+ depletion (1A8 Clone) and IL-4 neutralization ((IL-4 Neut.) or isotype control antibody (Control) one day prior and three days post inoculation with AF293 conidia via non-invasive intratracheal instillation (107 conidia/ 25μL). Statistical significance was determined using the log-rank (Mantel-Cox) test. N = 10 per experimental group.

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