The distribution patterns of binding motifs for co-occurring SF motifs vary among different SF pairs.
(A-B) Binding motifs for EXC-7 and SUP-12, and for FOX-1/ASD-1 and EXC-7 were more likely to be conserved across Caenorhabditis species if they were found closer together. The proportions of motif pairs where both motifs were conserved across species were plotted cumulatively, where pairs of motifs with inter-motif distances less than or equal to the x-axis values were included at each point. The black horizontal line represents the proportion of conserved motifs among all considered motif pairs. (C) While the distances between EXC-7 and MEC-8 recognition motifs fall within a wide range, the relative position of the EXC-7 motif as being upstream of the MEC-8 motif is conserved in other Caenorhabditis species. For each AS event with co-occurring EXC-7 and MEC-8 binding motifs, the position of a MEC-8 recognition motif was plotted at 0 on the x-axis of the density plot and the position of the co-occurring EXC-7 motif was plotted relative to that. A positive value indicates positioning of an EXC-7 motif downstream of a MEC-8 motif, while a negative value indicates positioning of an EXC-7 motif upstream of a MEC-8 motif. Significance was calculated using a binomial test (* = p < 0.05).