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The concentration dependent effect of AH6809 on nephrin, podocin, and CD2AP expression in TGF-β1 treated podocytes.

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posted on 10.05.2018, 18:04 by Jing Liu, Yi-de Zhang, Xiao-lan Chen, Xue-ling Zhu, Xu Chen, Jian-hua Wu, Nai-feng Guo

Podocytes were pretreated for 30 min with different concentrations (10−7,10−6 or 10-5mol/L) of AH6809 (n = 4 each dose), and were stimulated with 5 ng/ml TGF-β1 for 24 h. Nephrin, podocin, and CD2AP were determined at the mRNA and protein level.(A:RT-qPCR, B: Western blot). aP<0.05 compared to the control group, bP<0.05 compared to the TGF-β1 group.

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