The coimmunoprecipitation of Dsg and Dsc.
(A) Sections of skin equivalents were stained with hematoxylin and eosin (H&E) (B) Expression level of Dsg1 and Dsc1 was unaffected by the presence or absence of serine. Expression level of α-Tubulin was verified as a control for loading of same amount of the cell lysates in gel electrophoresis. (C) Immunoprecipitates by anti-Dsg1 antibody were analyzed by immunoblotting with anti-Dsg1 and anti-Dsc1. In the presence of serine, the amount of coimmunoprecipitated Dsc1 decreased. Bars indicate means ± SD of relative densitometric values of Dsc1 from the repeated experiments (n = 3). *P < 0.05 vs. the untreated control.