The androgen receptor can signal through Wnt/β-Catenin in prostate cancer cells as an adaptation mechanism to castration levels of androgens-3
figureposted on 2011-12-31, 13:27 authored by Liang Schweizer, Cheryl A Rizzo, Thomas E Spires, J Suso Platero, Qiuyan Wu, Tai-An Lin, Marco M Gottardis, Ricardo M Attar
Taken from "The androgen receptor can signal through Wnt/β-Catenin in prostate cancer cells as an adaptation mechanism to castration levels of androgens"
BMC Cell Biology 2008;9():4-4.
Published online 24 Jan 2008
PMCID:PMC2246119.thout Wnt3A 100 ng/ml for four days in 96 well plates. The cells were then labeled with [H]-thymidine and harvested to measure the thymidine incorporation. Cells that received no DHT treatment were used as control. The percentage of [H]-thymidine incorporation was calculated compared with control. (B). LNCaP and LNCaP-Flag-AR cells were plated in soft agar with no treatment as control or with 100 ng/ml Wnt3A, or 10 nM DHT. After approximately 4 weeks, colonies were fixed with 10% formaldehyde in PBS. A representative field of cells was photographed for each cell type, with or without treatment using bright-field microscopy. Upper panel LNCaP or LNCaP-Flag-AR cells received no treatment, while lower panel, LNCaP-Flag-AR cells were either treated with Wnt3A or DHT.