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The activity of the AtDRTS1 and AtDRTS2 promoters is cell cycle regulated in germinating seeds.

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posted on 08.06.2017 by Antonio Maniga, Stefania Ghisaura, Lara Perrotta, Maria Giovanna Marche, Rino Cella, Diego Albani

Fluorimetric analysis of GUS activity in transgenic lines harbouring the DRTS1 (A), DRTS2 (B) and DRTS2ΔE2F (C) promoter constructs was carried out on extracts obtained from germinating seeds incubated 72 h without (ctr) or with cell cycle inhibitors (Aph, Col). The results obtained with one representative line for each construct are presented. The analyses were carried out using three biological replicates. Treatment with colchicine lowers significantly the GUS activity of germinating seeds of each line. The bars show standard errors. *p<0.05, **p<0.01.