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The VER ligand PVF-1 is required for proper GLR-1 trafficking and behavior.

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posted on 09.02.2021, 18:38 by Eric S. Luth, Molly Hodul, Bethany J. Rennich, Carmino Riccio, Julia Hofer, Kaitlin Markoja, Peter Juo

(A) Nose touch responses for the indicated mutants and transgenic strains are shown. (B) Nose touch responses for WT worms and pvf-1 mutants with and without a 30°C heat shock for 3 hours and/or hsp-16.2::PVF-1 rescue transgene as indicated. Rates of thrashing (C) and aldicarb-induced paralysis (D) are shown. Mean ± SEM are shown (n ≥ 20 worms from 3 experiments). Values that differ significantly from wild type are indicated by asterisks above each bar, whereas other comparisons are marked by brackets. *p ≤ 0.05 ANOVA, Dunnett’s multiple comparison test. Representative images (E-G) and quantification (H) of the SEP/mCherry ratio for the indicated genotypes are shown. # marks fluorescence from a co-injection marker. Mean ± SEM are shown (n ≥ 23 worms from 3 experiments). Values that differ significantly from wild type are indicated by asterisks above each bar, whereas other comparisons are marked by brackets. *p ≤ 0.05, ANOVA, Tukey’s multiple comparison test. (I) Confocal image showing the accumulation of PVF-1::GFP in vesicles inside a coelomocyte (marked by unc-122p::RFP). Merge images have been false colored magenta (mCherry) and green (SEP).

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