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The REL2 is essential in rapamycin mediated increasing resistance to Plasmodium infection.

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posted on 2021-02-24, 18:44 authored by Yuebiao Feng, Lu Chen, Li Gao, Li Dong, Han Wen, Xiumei Song, Fang Luo, Gong Cheng, Jingwen Wang

Western blot analysis of TEP1 in fat bodies of rapamycin-treated (A) and dsRNA- treated (B) mosquitoes 24 hpi. The blot was probed with the anti-TEP1 polyclonal antibody. An. stephensi ACTIN was used as the loading control. The bar chart represents relative quantification of signal intensity from at least two independent replicates determined by ImageJ software. Error bars indicate standard errors (n ≥ 2). (C) Whole-mount staining of TEP1 (red) in fat bodies of rapamycin-treated (RAPA) and control (Control) mosquitoes 24 hpi. Nuclei were stained with DAPI (blue). Images are representative of three independent experiments. Scale bars = 50 μm. Influence of REL2 on gene expression of SPCLIP (D), and TEP1 (E) in rapamycin-treated mosquitoes. Expression of target genes was normalized to the reference gene S7. Relative gene expression in treated mosquitoes was normalized to that of dsGFP controls. Error bars indicate standard errors (n = 9). Results from one of three independent experiments are shown. (F) Influence of TEP1 on parasite infection. Data were pooled from three independent experiments. (G) Influence of REL2 on vector competence in rapamycin-treated mosquitoes. Data were pooled from at least two independent experiments. Significance was determined by Student’s t-test in (A), (B), (D) and (E), and by a Mann-Whitney test in (F, G). *P<0.05, **P<0.01; NS, not significant.

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