The EFGR immunostaining of lamellae from control (non-laminitic) horses.
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A: The parabasal cells (*) of each secondary epidermal lamella (SEL) showed positive immunostaining. The cytoplasm of lamellar epidermal basal cells (↓), the cell membrane of lamellar basal cells (adjacent to the basement membrane) and the keratinised axis of the primary epidermal lamella (PEL) stained lightly. Staining was absent in the primary dermal lamellae (PDL). B: At higher magnification the positive staining of the parabasal cells is diffuse, but particularly evident around nuclei (*). The cytoplasm of lamellar epidermal basal cells stained lightly and was absent adjacent to nuclei (↓) except where it extended to the base of the cell. Staining was absent in secondary dermal lamellae (SDL). C: Basal cell staining was absent adjacent to nuclei except where it extended to the cell base (black ↓), contiguous with the dermo-epidermal junction. There was no immunostaining of the basement membrane. A: Bar = 100 μm, B: Bar = 50 μm, C: Bar = 25 μm.