The CZA97.012 SOSIP.664-trimer structure.

figure

posted on 2023-10-30, 18:00 authored by Rajesh P. Ringe, Philippe Colin, Gabriel Ozorowski, Joel D. Allen, Anila Yasmeen, Gemma E. Seabright, Jeong Hyun Lee, Aleksandar Antanasijevic, Kimmo Rantalainen, Thomas Ketas, John P. Moore, Andrew B. Ward, Max Crispin, P. J. Klasse

A. The 3.4-Å cryo-EM reconstruction of CZA97.012 SOSIP in complex with 3BNC117 Fab is colored as labeled. B. The atomic model of the CZA97.012 complex highlights gp120 variable regions. C. Alignment of all 6 Env chains (3-gp120, 3-gp41) of BG505+3BNC117 (PDB 5v8m) onto the CZA97.012+3BNC117 model. Root- mean-square deviation (r.m.s.d.) was calculated between backbone Cα atoms. D. Map (contoured at 5σ) and model of the CZA97.012 V4-β19 region and surrounding N-linked glycans are shown. E. Interprotomer distances between R166 Cα in CZA97.012+3BNC117, BG505+3BNC117 (PDB 5v8m), and BG505+3BNC117+PGT145 (PDB 5v8l) are given. F. Alignment of BG505 SOSIP.664+35O22 (PDB 4tvp (112)) onto CZA97.012+3BNC117, with a focus on the gp41 part of the epitope. G. Map (contoured at 5σ) and model of the first two GlcNAc residues of at N160 and surrounding residues of one protomer of CZA97.012 SOSIP.664.