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Figure S1 - The Axe-Txe Complex of Enterococcus faecium Presents a Multilayered Mode of Toxin-Antitoxin Gene Expression Regulation

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posted on 03.09.2013, 02:16 by Lidia Boss, Łukasz Labudda, Grzegorz Węgrzyn, Finbarr Hayes, Barbara Kędzierska

Neither Axe-Txe proteins nor other proteins in the E. coli extract bound detectably to a fragment bearing the wild-type paxe promoter. A 126 bp 5’ biotinylated fragment that includes paxe was subjected to EMSA. DNA samples were incubated with the different crude extracts concentrations of E. coli BL21(DE3) harbouring pET22at_axe-txe plasmid (left to right): 0, 1.25, 2.5, 5, 10, 12.5 and 25 µg/ml for 20 min at 220C and analyzed by a native 5% PAGE. Reactions were processed as outlined in Materials and Methods.