Figure S1 - The Axe-Txe Complex of <i>Enterococcus faecium</i> Presents a Multilayered Mode of Toxin-Antitoxin Gene Expression Regulation
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posted on 2013-09-03, 02:16 authored by Lidia Boss, Łukasz Labudda, Grzegorz Węgrzyn, Finbarr Hayes, Barbara Kędzierska<p><b>Neither Axe-Txe proteins nor other proteins in the <i>E. coli</i> extract bound detectably to a fragment bearing the wild-type p<sub><i>axe</i></sub> promoter. </b>
A 126 bp 5’ biotinylated fragment that includes <i>p</i><sub><i>axe</i></sub> was subjected to EMSA. DNA samples were incubated with the different crude extracts concentrations of <i>E. coli</i> BL21(DE3) harbouring pET22at_axe-txe plasmid (left to right): 0, 1.25, 2.5, 5, 10, 12.5 and 25 µg/ml for 20 min at 22<sup>0</sup>C and analyzed by a native 5% PAGE. Reactions were processed as outlined in Materials and Methods.</p> <p>(TIF)</p>
