Test of virus particle recovery after column washes with and without Tween 20 on anti-HLA-DR and anti-CD16 captures of DNase-treated cultured HIV_ADA-M virus.

RNA amplifications shown for anti-HLA-DR columns (green lines) and anti-CD16 columns (blue lines), and column with no antibody present (red line). Curves shown for columns either washed three times without Tween 20 or washed with buffer containing 1% Tween 20 as per protocol. Amplification from the no-Ab column (red line) demonstrates particles are non-specifically retained on columns in the absence of Tween 20.

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