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Tat is involved in S. Tm-induced colitis.

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posted on 31.10.2018, 18:35 by Mayuka Fujimoto, Ryosuke Goto, Riku Hirota, Masahiro Ito, Takeshi Haneda, Nobuhiko Okada, Tsuyoshi Miki

(A-F) Streptomycin-treated C57BL/6 mice (n = 9 or 10 per group) were infected intragastrically for 3 days with 5×107 CFU S. Tm wild-type strains SL1344 (wt) or tatC mutant (tatC). (A) S. Tm loads in feces at 1 and 3 days postinfection (dpi). (B) S. Tm loads in cecum at 3 dpi. (C) S. Tm loads in mesenteric lymph node (mLN) at 3 dpi. (D) S. Tm loads in spleen at 3 dpi. (E) Cecal pathological score in H&E-stained cecal tissue section. (F) Fecal lipcalin-2 ELISA. (G-J) Streptomycin-treated C57BL/6 mice (n = 7) were infected intragastrically for 4 days with 1:1 mixture (total 5×107 CFU) of S. Tm strains wt and tatC. (G) CI of S. Tm loads in feces at 1 to 4 dpi. (H) CI of S. Tm loads in cecum at 4 dpi. (I) CI of S. Tm loads in mLN at 4 dpi. (J) CI of S. Tm loads in spleen at 4 dpi. Bar indicates median. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; Mann-Whitney U test.

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