Tagging of PBANKA_0524300 (IPIS2), PBANKA_0623100 (IPIS3), and PBANKA_1400700 with mCherry.
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posted on 2022-09-20, 17:42 authored by Julie-Anne Gabelich, Josephine Grützke, Florian Kirscht, Oliver Popp, Joachim M. Matz, Gunnar Dittmar, Melanie Rug, Alyssa Ingmundson(A) Schematic of the endogenous C-terminal tagging with mCherry. Constructs were integrated into parasite genomic DNA through a single crossover homologous recombination event. Primers used for the genotyping are indicated as grey arrows. (B) Diagnostic PCR with gDNA of P. berghei wild-type parasites (top panel) and transgenic parasites obtained after transfection (bottom panel) confirmed the integration of the linearized vector into the genome. (C) PBANKA_0524300-mCherry, PBANKA_0623100-mCherry, and PBANKA_1400700-mCherry are detected in punctate structures in the cytoplasm of fixed infected erythrocytes.
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successful parasite infectionerythrocytic membrane compartmentscharacterize protein componentshost membrane remodelingdiv >< pdata describing proteinsrich protein familythree candidate ibis1conserved across multiple>- infected cells>- infected erythrocytesplasmodium berghei required across speciesinfected cellsplasmodium p berghei infected erythrocyteserythrocytes infectedprotein exportrich domainhost erythrocyteinfected hostsidentified proteinsexported proteinscontaining proteinsvivo therefore failstage growthptex (<particular failfacilitate interactionsefficient sequestrationefficient bloodeffectively sequestercomponent exp2circulating bloodbind cd36better understandalso report
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