Tagging of PBANKA_0524300 (<i>IPIS2</i>), PBANKA_0623100 (<i>IPIS3</i>), and PBANKA_1400700 with mCherry.

<p>(A) Schematic of the endogenous C-terminal tagging with mCherry. Constructs were integrated into parasite genomic DNA through a single crossover homologous recombination event. Primers used for the genotyping are indicated as grey arrows. (B) Diagnostic PCR with gDNA of <i>P</i>. <i>berghei</i> wild-type parasites (top panel) and transgenic parasites obtained after transfection (bottom panel) confirmed the integration of the linearized vector into the genome. (C) PBANKA_0524300-mCherry, PBANKA_0623100-mCherry, and PBANKA_1400700-mCherry are detected in punctate structures in the cytoplasm of fixed infected erythrocytes.</p> <p>(TIF)</p>