Fig 7.tif (4.32 MB)
Download file

TUNEL positive cells in the zebrafish retina after exposure to MNU.

Download (4.32 MB)
posted on 23.11.2016, 17:34 by Christoph Tappeiner, Ellinor Maurer, Pauline Sallin, Thomas Bise, Volker Enzmann, Markus Tschopp

In uninjured zebrafish retina there are merely no TUNEL positive cells. Three days after exposure to 150 mg/l MNU, both the non-inhibited (0.1% dimethyl sulfide, DMSO) and the inhibited group (small molecule inhibitor SB431542) show a considerable amount of TUNEL positive cells (green) in the outer nuclear layer (ONL) and to a lesser degree in the inner nuclear layer (INL). Cell nuclei are stained with DAPI (blue). Scale bar indicates 50 μm. GC: ganglion cells.