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TCRβ expression correlates with enhanced phagocytosis of parasites by macrophages.

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posted on 25.07.2018, 17:50 by Miranda S. Oakley, Joanna K. Chorazeczewski, Maya Aleshnick, Vivek Anantharaman, Victoria Majam, Bhavna Chawla, Timothy G. Myers, Qin Su, Winter A. Okoth, Kazuyo Takeda, Adovi Akue, Mark KuKuruga, L. Aravind, Sanjai Kumar

A: Splenocytes and parasitized red blood cells (pRBCs) were isolated from C57BL/6 mice on day 3 post-infection with Plasmodium berghei ANKA. A 1:1 ratio of splenocytes (labeled with macrophage and T lymphocyte markers) and pRBCs (labeled with CellTrace®) were then incubated for 90 minutes to assess the effect of TCRβ expression by Ly6GCD11bhighF4/80+ macrophages on phagocytosis in an in vitro assay. B: Expression of TCRβ correlates with enhanced phagocytosis of pRBCs by macrophages. n = 3 for each phagocytosis assay. Results are presented as mean ± standard deviation and are a replicate of three independent experiments. Significant difference between TCRβ+ versus TCRβ macrophages is indicated as **P < 0.01. P−value was calculated using the Mann Whitney U test.