TCRβ expression correlates with enhanced phagocytosis of parasites by macrophages.
A: Splenocytes and parasitized red blood cells (pRBCs) were isolated from C57BL/6 mice on day 3 post-infection with Plasmodium berghei ANKA. A 1:1 ratio of splenocytes (labeled with macrophage and T lymphocyte markers) and pRBCs (labeled with CellTrace®) were then incubated for 90 minutes to assess the effect of TCRβ expression by Ly6G−CD11bhighF4/80+ macrophages on phagocytosis in an in vitro assay. B: Expression of TCRβ correlates with enhanced phagocytosis of pRBCs by macrophages. n = 3 for each phagocytosis assay. Results are presented as mean ± standard deviation and are a replicate of three independent experiments. Significant difference between TCRβ+ versus TCRβ− macrophages is indicated as **P < 0.01. P−value was calculated using the Mann Whitney U test.