Survival distribution, infant mice and hamsters inoculated by the IC route.

A. 8-day-old ICR mice. Twenty μL of PHV02 at graded doses or YF 1DD vaccine were inoculated into 2 litters (10 suckling pups/litter). The doses injected in 20 μL were 100,000, 1000, or 10 pfu for PHV02 groups and 640 pfu for YF 17DD. Animals were observed for 21 consecutive days and clinical signs and deaths recorded. All mice died in the intermediate and high dose groups, 85% died in the low dose (10 pfu) group and 70% died in the reference group receiving 640 pfu of YF17DD. B. 8-day-old Golden hamsters. Twenty μL of PHV02 at graded doses or YF 17DD were inoculated into 2 litters (8-10 suckling pups/litter). The doses injected in 20 μL were 1000 or 10 pfu for PHV02 groups and 640 pfu for YF 17DD. All infant hamsters died, but the YF 17DD animals had a statistically longer survival time (see text). Since all animals in the test article groups died by Day 7, the study was terminated at that point.