Supplementary Material D; Melanisation and Flourescence Index Photos

File contains example pictures used to index melanisation and flourescence in Galleria infection assays, adapted from [Kay et al, 2019].

Fluorescence was assessed using a M165 FC Leica stereoscope equipped with a Leica EL6000 external light source for fluorescence excitation and visualized using the GFP plus filter [480/40 nm (460–500 nm); Leica Microsystems (UK) limited].

Kay, S., Edwards, J., Brown, J., & Dixon, R. (2019). Galleria mellonella Infection Model Identifies Both High and Low Lethality of Clostridium perfringens Toxigenic Strains and Their Response to Antimicrobials. Frontiers in Microbiology, 10, 1281. https://doi.org/10.3389/fmicb.2019.01281