Supplementary Figure S2. Adrenal Cortex Dysgenesis in Nr5a1-Cre;Nedd4flox/flox Mice A) Section immunofluorescence on 14.5 dpc Nr5a1-Cre;Nedd4flox/floxadrenal glands alongside Cre-negative littermate controls stained for NEDD4 (green) and NR5A1 (magenta) merged with nuclear stain DAPI (blue). B) Section immunofluorescence on Nr5a1-Cre;Nedd4flox/floxand Cre-negative littermate controls at 14.5, 16.5 and 18.5 dpc stained for the steroidogenic, adrenal cortex marker NR5A1 (green) and the chromaffin cell, adrenal medulla marker TH (magenta) alongside nuclear stain DAPI (blue). Scale bars = 100 μm.
Funding
Australian Research Council Discovery Grant (150101448),
Monash University Biomedicine Discovery Institute Early Career Award,
National Health and Medical Research Council of Australia Investigator Grant (GNT 2007739),
Swiss National Science Foundation under grants 31003A_173070 and 310030_200316.