Supplementary Figure S1: Ablation of NEDD4 in Somatic Cells of the Testis
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posted on 2025-06-11, 05:19 authored by Simon WindleySimon Windley, Yasmine Neirijnck, Diana VidovicDiana Vidovic, Quenten SchwarzQuenten Schwarz, Sharad KumarSharad Kumar, serge nefserge nef, DAGMAR WILHELMDAGMAR WILHELMSupplementary Figure S1. Ablation of NEDD4 in Somatic Cells of the Testis
A) Section immunofluorescence on 11.5 dpc XY Nr5a1-Cre;Nedd4Flox/Flox testes alongside Cre-negative XY controls stained for NEDD4 (green) and germ cell marker DDX4 (magenta). Scale bar = 100 μm. B) RT-qPCR analysis of Nedd4 expression at 14.5 dpc on XY Nr5a1-Cre;Nedd4Flox/Flox gonads (blue, n=5) and XY Cre-negative littermate controls (grey, n=5). Values are normalized to Sdha and are expressed as fold change relative to controls. Mean ± SEM; t-test; * = p < 0.05. C) Section immunofluorescence on 14.5 dpc XY Nr5a1-Cre;Nedd4Flox/Flox testes alongside Cre-negative XY controls stained for NEDD4 (green) and Sertoli cell marker SOX9 (magenta). Scale bar = 100 μm.
