Supplementary Figure 3. Traction force microscopy quantification of TGFβ stimulated IMR-90 cells with 5 µM siRNA (non-targeting, ACTA2, CNN1, TAGLN).
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posted on 2021-09-23, 18:43 authored by Patrick LinkPatrick Link, haak.andrew@mayo.edu, Tschumperlin.daniel@mayo.edu, choi.kyoung@mayo.edu, diazespinosa.ana@mayo.edu, Dakota.jones@pennmedicine@upenn.edu, gao.ashley@mayo.eduA) IMR-90 cells treated for 72 hours +/- 2 ng/ml TGFβ1 in the presence of 5 µM siRNA (non-targeting, ACTA2, CNN1, TAGLN) were passaged onto microsphere and collagen-coated polyacrylamide substrates with an Elastic modulus of 25 kPa overnight before traction-force measurements. Points are individuals cell measurements from 2 independent experiments. B-D) qPCR confirmation of mRNA knockdown for ACTA2 (B), CNN1 (C), and TAGLN (D).
