Supplementary

FIGURE S1. γ-ENaC is expressed in human leukemia K562 but not in MOLT-4 cell lines. Immunofluorescent staining with specific antibodies detected γ-ENaC (red) in K562 cells. Cell nuclei stained with DAPI (blue). Scale bar is 50 μm. No staining was observed in MOLT-4 cells. The rectangles show cells that are 2X zoomed.

FIGURE S2. Effect of amiloride on the migration and viability of K562 leukemia cells. (A) Cell viability was determined using the MTT detection kit. (B) Incubation with amiloride (2 mM) alone and amiloride with α-chymotrypsin (chymo, 5 μg/mL) were performed during 4 h. Data are normalized to control (cntrl) and are presented as mean±SEM, data were collected from N=3 independent experiments with n=3 data points for each experimental condition. **** - p <0.0001, ns - non-significant. The data were compared using Student’s unpaired t-test.