Supplemental Figure 1Development of Novel Technology for the Visualization and Quantitation of Angiogenesis and the Alveolar-Capillary Network in a Mouse Model of Fibrosis

Supplemental Figure 1. Wimasis Quantitative Pilot Image Analysis of the Alveolar-Capillary Network. A.Image Z-stacks were uploaded to Wimasis for analysis. The pulmonary alveolar-capillary network was analyzed for WT PBS vehicle or day 14 bleomycin treated groups with image statistics generated using B.skeletonized reconstructions and C. vessel diameter heat maps to quantitate microvessels. Scale bar = 50µm.D&E. Quantitation of Vessel Volume and Diameter (n=3,3 mice/group with 3 images analyzed per mouse). Each mouse triplicate is depicted and represented by a shape. The average of the 3 mice per group were analyzed to define the mean and standard error per group. Significance was tested by nonparametric Wilcoxon/Kruskal Wallis and a chi square approximation in JMP version 16. Significance was defined as p-value <0.05*.